ABSTRACT
OBJECTIVE：To investigate the effects of dihydroartemisinin （DHA）on the metabolism of amino acid metabolites in human hepatocellular carcinoma cells Huh 7 and BEL- 7402，and to provide theoretic basis for clarifying the mechanism of DHA regulating the metabolism of hepatocellular carcinoma cells. METHODS ：CCK-8 method was taken to detect the effect of different concentrations of DHA （12.5，25，50，100 µmol/L）treating for 24，48，72 h on the two kinds of cells. Two kinds of cells were divided into control group and administration group （DHA，25 µmol/L），and then cultured with drug-free or drug-containing medium for 24 h，operated in parallel for three times. After derivatization of cell samples in each group ，GC-MS method was used to detect the content of amino acid metabolites ，combined with SIMCA-P software analysis and compound library comparison ，the differential metabolites in two kinds of cells were screened out. The pathway enrichment analysis of differential metabolism was conducted with Metaboanalyst 4.0 software. RESULTS ：Compared with control group ，the contents of glutamine ，glutathione， phenylalanine，fumaric acid and taurine were trending down in Huh 7 or BEL- 7402 cells. There were 28 and 29 differential metabolites obtained from the above two kinds of cells ，and 10 of them were common differential metabolites ，including glutamine，glutathione，taurine，fumaric acid ，phenylalanine，etc. The differential metabolites were enriched in 8 and 6 pathways respectively. The common enrichment pathways were amino acid-tRNA biosynthesis ，aspartate-alanine-glutamate metabolism ， nitrogen metabolism ，phenylalanine metabolism and pentose phosphate pathway ，etc. CONCLUSIONS ：DHA can significantly reduce the activities of Huh 7 cells and BEL- 7402 cells，and the contents of glutamine ，glutamic acid ，glutathione and phenylalanine，etc. It may regulate the growth of the two kinds of cells by influencing the mechanism of aspartic acid- alanine-glutamate metabolic pathway ，etc.
ABSTRACT
Seven sinapine analogs (6a-6g) were synthesized using cinnamon acid or benzoic acid and their derivatives as starting materials, which obtained from substituted benzaldehyde and malonate. The structures of target compounds were characterized by IR, 1H NMR and elemental analysis. The effects of compounds 6a-6g on the smooth muscle of intestine isolated from rabbit were studied, and the experimental results showed that compounds 6a, 6d and 6g had diastolic action, while 6f had contractile action.
ABSTRACT
Objective To observe the immune regulatory effect of Marasmius androsaceus (MA) polysaccharide in normal mice. Methods The experimental mouse was injected with high,middle,and low dosages of marasmius androsaceus polysaccharide(5,10,20 mg?kg-1?d-1) in the abdomen (i.p.) ,and the comparison group was given the same volume of saline water i.p. once a day. After treatment for 14 consecutive days,the blood cell count instrument was used to measure the mice hemogram,the phagocytic function of peritoneal macrophage and the ability of carbon granular clearance were observed.,the ability of the spleen lymphocytes proliferation was tested by MTT,and the hemolysin production test was used to evaluate the influence of MA polysaccharide on specific humoral immunity in mice. Results Marasmius androsaceus polysaccharide at middle and high dosages can increase the number of lymphocytes and moncytes in normal reference,enhance the phagocytic function of peritoneal macrophage,promote the carbon granular clearance,accelerate the proliferation of T lymphocyte,enhance the specific humoral imnune function. Conclusion Marasmius androsaceus polysaccharide has certain potentiating effect on the phagocytic function and immunity regulation.